Work

Antcin K ameliorates cardiotoxin-induced skeletal muscle injury and inflammation via IL-10 regulation

Year: 2025

Type: article

Abstract: Background: Skeletal muscle, functioning as an endocrine organ, produces a variety of molecules that contribute to the pathophysiology of sarcopenia, leading to muscular injury and inflammation.Antcin K, a bioactive compound derived from Antrodia cinnamomea and used in traditional Chinese medicine for its anti-inflammatory properties, was evaluated in this study with the aim of assessing its effects on resisting the progression of sarcopenia both in vitro and in vivo.Methods: Cardiotoxin (CTX)-induced muscle injury and the treatment of Antcin K in C2C12 cells were both used for RNA sequencing and ingenuity pathway analysis.We also stably cloned an IL-10 knockdown (IL-10 -/+ ) C2C12 cell line for the effects of Antcin K treatment on CTX-induced muscle injury.CTX-induced muscle injury in a mouse model.Results: Antcin K ameliorated the CTX-induced muscle injury and inflammation in myoblasts and differentiated myocytes.Bioinformatics analysis results demonstrated the ability of Antcin K to modulate inflammation and enhance myogenesis via upregulated IL-10.Antcin K enhances IL-10 production via the PI3K/Akt signaling pathways.For the in vivo results, Antcin K protects against CTX-induced skeletal muscle inflammation and injury.Conclusion: Antcin K ameliorated CTX-induced muscle injury and inflammation through PI3K and Akt and upregulated IL-10 in vitro.The CTX-induced injury mouse model was rescued by intraperitoneal injection of Antcin k in vivo.Antcin K shows promise as a prospective candidate for the development of an innovative treatment for muscular injury, with significant implications for sarcopenia.

Source: International Journal of Biological Sciences

DOI: 10.7150/ijbs.107343