Work

Episomal virus maintenance enables bacterial population recovery from infection and promotes virus–bacterial coexistence

Year: 2025

Type: article

Abstract: Viruses are ubiquitous in aquatic environments with total densities of virus-like particles often exceeding 107/ml in surface marine oligotrophic waters. Hypersaline environments harbor elevated prokaryotic population densities of 108/ml that coexist with viruses at even higher densities, approaching 1010/ml. The presence of high densities of microbial populations and viruses challenge traditional explanations of top-down control exerted by viruses. At close to saturation salinities, prokaryotic populations are dominated by Archaea and the bacterial genus Salinibacter. In this work we examine the episomal maintenance of a virus within a Salinibacter ruber host. We found that infected cultures of Sal. ruber M1 developed a population-level resistance and underwent systematic and reproducible recovery post infection that was counter-intuitively dependent on the multiplicity of infection, where higher viral pressures led to better host outcomes. Furthermore, we developed a nonlinear population dynamics model that successfully reproduced the qualitative features of the recovery. Together, experiments and models suggest that episomal virus maintenance and lysis inhibition enable host-virus co-existence at high viral densities. Our results emphasize the ecological importance of exploring a spectrum of viral infection strategies beyond the conventional binary of lysis or lysogeny.

Source: The ISME Journal

DOI: 10.1093/ismejo/wraf066

Authors: Rodrigo Sanchez-Martinez, Akash Arani, Mart Krupovic, Joshua S Weitz, Fernando Santos, Josefa Antón