FIGURE 2 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma
Year: 2025
Type: other
Abstract: EBV is preserved in NPC268 and can be induced into lytic infection. A, EBER staining was positive in early and late passage of NPC268, but LMP1 and BZLF1 protein expressions were lost in the late passage. Scale bar: 100 µm. B, EBV latent and lytic gene expression profiles in NPC268 in comparison with other EBV-positive NPC cell lines. C, EBV CN in NPC268 has reduced following prolonged culture but stabilized at later passages, retaining about approximately 13 copies of EBV per cell. D, EBV-FISH assay confirming presence of EBV-positive cells in late passage of NPC268. E, EBV lytic genes-encoded protein expression in NPC268 induced by different methods. EBV-negative Akata cells infected with EBV derived from NPC268 expresses Zta and Ea-D, confirming infectious EBV production. NPC43 treated with TPA for 48 hours was used as positive control. F, qPCR analysis of the EBV-negative Akata cells showed substantial upregulation of various latent and lytic genes postinfection with the NPC268-derived EBV. Data are shown as mean ± SD (n = 2 biological repeats).
Authors: Annie Wai Yeeng Chai, Shi Mun Yee, Hui Mei Lee, Norazlin Abdul Aziz, Pei San Yee, Marini Marzuki, Ka Wo Wong, Alan K.S. Chiang, Larry Ka-Yue Chow, Wei Dai, Teng Fei Liu, Lu Ping Tan, Alan Soo Beng Khoo, Kwok Wai Lo, Paul V.H. Lim, Pathmanathan Rajadurai, Howard Lightfoot, Syd Barthorpe, Mathew J. Garnett, Sok Ching Cheong
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4409367804