Work

Elucidating the synergistic role of hybrid peptide from Burkholderia cepacia enzymes in biodegradation of polycyclic aromatic hydrocarbons

Year: 2025

Type: article

Abstract: Polycyclic aromatic hydrocarbons (PAHs) are hazardous environmental contaminants emerging from industrial activities and fossil fuel combustion, posing risks to human health and ecosystems. Biodegradation offers a sustainable approach to mitigating PAH pollution, and here we investigated the efficacy of a peptide hybrid of laccase and O-methyltransferase enzymes from the bacterium Burkholderia cepacia in PAH degradation. Both enzymes demonstrated stability with an instability index below 40, indicating suitability for environmental application. Following active site prediction, the 3D structure of the peptide hybrid, consisting of 71 amino acids, was modelled using trRosetta, achieving a high-quality structure with an ERRAT score above 97%. Further bioinformatic analysis confirmed the hybrid's non-allergenic and non-virulent properties. Molecular docking studies revealed a robust binding affinity above - 9 kcal/mol, highlighting this peptide hybrid's potential for effective PAH degradation and suggesting its promise as an eco-friendly bioremediation agent for PAH-contaminated sites.

Source: Scientific Reports

DOI: 10.1038/s41598-025-97007-1