Work

A multifunctional trap-capture-kill antibacterial system for enhanced wound healing via modified decellularized mushroom aerogels

Year: 2025

Type: article

Abstract: Wound infections are prevalent and can result in prolonged healing times. In this study, we referred to the “trap-capture-kill” antibacterial strategy to create a wound dressing (DS/PDA@GO-L) by coupling graphene oxide (GO) with lysine and coating it onto the decellularized mushroom stem (DS) using polydopamine (PDA). The mechanism of action of the bacteria-killing process involves lysine chemotaxis and the siphoning effect of DS aerogel , with the process of killing the bacteria being initiated via near-infrared photothermal treatment. In vitro studies demonstrated that DS/PDA@GO-L exhibited excellent blood and cell compatibility, while in vivo experiments revealed its remarkable efficacy in combating bacterial infections. Specifically, the combination of DS/PDA@GO-L with photothermal therapy led to the elimination of over 95 % of S. aureus , E. coli , and Pseudomonas aeruginosa . Furthermore, the aerogel , when used in conjunction with photothermal therapy, significantly reduced bacterial infection at the wound site and accelerated wound healing . During the wound's proliferative phase , it notably enhanced vascularization and extracellular matrix deposition. Furthermore, immunohistochemical staining revealed that bacterial clearance led to a reduction in pro-inflammatory responses and a decrease in the expression of pro-inflammatory cytokines, thereby restoring the wound's inflammatory environment to a pro-regenerative state. Taken together, the developed DS/PDA@GO-L holds great potential in the field of infected skin wound healing.

Source: Bioactive Materials

DOI: 10.1016/j.bioactmat.2025.03.022