Work

Determination of Human Papillomavirus Serotypes in Endocervical Samples from Patients with Normal Cytology from a Mexican Population by Polymerase Chain Reaction-restriction Fragment Length Polymorphism

Year: 2025

Type: article

Abstract: ABSTRACT: Human papillomavirus (HPV) is associated with cervical cancer (CC). Genotypes 16 and 18 are high-risk (HR) factors for CC. The Papanicolaou (Pap) identifies the morphological changes for CC. However, HPV is presented in normal Pap smears. This study aimed to estimate the prevalence of HPV in patients with normal cytology and a cross-sectional study was conducted among 141 women with normal cytology. DNA was extracted from a cervical swab. The viral genome was screened by polymerase chain reaction-restriction fragment length polymorphism, using MY09/11 and L1C1/L1C2 oligonucleotides. A bivariate analysis of the main risk factors was performed. Odds ratios (ORs) and 95% confidence intervals (CIs) were obtained from multiple logistic regression models. HPV prevalence increased to 16.3% (95% CI) when L1C1/L1C2 were used. Genotypes 11, 13, 33, and 59 were detected; 33 and 59 are HR factors for CC. The number of sexual partners is associated with HPV, OR 5.2 (P < 0.05). The regional HR variations should be considered in CC prevention.

Source: Indian Journal of Public Health

DOI: 10.4103/ijph.ijph_66_24

Authors: Joel Jahaziel Díaz-Vallejo, Esmeralda Lagunes-Baez, Daniela Córdoba-Colorado, Magda Olivia Pérez-Vásquez, Luz Irene Pascual-Mathey

Institutions: Universidad Veracruzana, Mexican Social Security Institute

Language: English

Topic: Cervical Cancer and HPV Research

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $48