Work

Conserved specificity of extracellular wastewater peptidases revealed by multiplex substrate profiling by mass spectrometry

Year: 2025

Type: article

Abstract: Peptide-based chemicals are promising for numerous applications including home and personal care and medical treatments. To better understand and control the environmental fate of peptide-based chemicals, in-depth knowledge on the specificity of wastewater peptidases is needed. Here, we employed multiplex substrate profiling by mass spectrometry to obtain specificity profiles of extracellular peptidases derived from influent and aeration tanks of three full-scale wastewater treatment plants. Specificities were confirmed by fluorogenic peptidase substrates. Our results revealed highly similar specificity profiles across wastewater treatment plants. We found that hydrolysis by extracellular wastewater peptidases is favored when positively charged amino acid residues surround the cleavage site and disfavored when negatively charged amino acid residues surround the cleavage site. Supplementary Information: The online version contains supplementary material available at 10.1007/s10311-025-01834-7.

Source: Environmental Chemistry Letters

DOI: 10.1007/s10311-025-01834-7

Authors: Natalie Wichmann, Josephine Meibom, Tamar Kohn, Michael Zumstein

Institutions: University of Vienna, Swiss Federal Institute of Aquatic Science and Technology, École Polytechnique Fédérale de Lausanne

Language: English

Topic: Pharmaceutical and Antibiotic Environmental Impacts

Subfield: Pollution

Field: Environmental Science

Domain: Physical Sciences

SDG: Clean water and sanitation

Open Access status: hybrid

Estimated APC paid: $3,190