Investigating The Relationship Between Biofilm Formation and Antibiotic Resistance Patterns of Escherichia Coli Isolated from Various Sources
Year: 2025
Type: article
Abstract: Bacteria can enhance their endurance by joining lifeless surfaces or tissues and introducing multicellular populations encased in a defensive extracellular lattice known as biofilm. Inside this element, microorganisms are exceptionally resistant and impervious to antibiotics. The ongoing work is meant to give more accurate information on the issue by looking at the biofilm-formation ability of Escherichia coli isolates in association with antibiotic resistance, as well as a total survey of the subject. Methods: For our situation study, a sum of 240 specimens were gotten from different sources, including urine (74), stool (62), tooth decay (53), and burns (51). The morphology of isolates was observed following 24 hours, and testing for antimicrobial vulnerability was done by the Kirby-Bauer disk dispersion method. Biofilm production of the isolates was identified by the tube methods, microtiter plate (MTP), and Congo red agar. Of the 240 separated isolates, just 20 were Escherichia coli, and every one of the 20 (100%) were positive for biofilm creation. Biofilms let microbes improve their defenses, guaranteeing their security from ecological hazards and harmful synthetics. Bacterial biofilms are connected to resistance to antibiotics, as seen by impressively expanded degrees of protection from aztreonam, rifampin, ertapenem, cefixime, ceftazidime, ceftriaxone, and trimethoprim/sulfonamides (P-value: 0.0013). The current study demonstrated that the E. coli clinical isolate from sick people in al-Najaf, Iraq, showed a positive link between the antibiotic resistance and biofilm formation; hence, more studying is required to completely understand the connection between antibiotic resistance and biofilm formation, which will help the advancement of imaginative treatment strategies
Author: زهراء هادي
Institution: University of Kufa
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
Funder: University of Kufa
OpenAlex ID: W4409398007