Year: 2025
Type: preprint
Abstract: Cellular gene expression varies in different physiological and pathological conditions. Analysis of differential gene expression enables researchers to understand the cellular changes associated with physiological or pathological conditions. During gene expression analyses, researchers calculate the transcripts expressed from a gene under that gene's name, assuming only a single mRNA is expressed by that gene. However, this assumption is biologically inaccurate, but it is often preferred to avoid the complex analyses of isoforms. Most mammalian genes express more than three mRNAs, which encode various proteins or act as noncoding RNAs. We have previously addressed the molecular basis mRNA isoform formation and detection strategies. In this review article, we have discussed the physiological and pathological roles of mRNA isoforms. Any specific cell may express different isoforms of mRNA from a specific gene depending on its differentiation state. Different isoforms can be expressed from a single gene at various stages of development and during aging. The same cell may also express mRNA isoforms related to pathological conditions. Summarized findings highlight the importance of detecting mRNA isoforms over conventional gene identification.
Source: Preprints.org
Authors: Sharmin Shila, Elizabeth Bahadursingh, Kevin Vo, Vinesh Dahiya, Patrick Fields, M.A. Karim Rumi
Institution: University of Kansas Medical Center
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4409400317