Work

Aspects of gene expression analysis in small–volume blood samples using the real–time PCR

Year: 2025

Type: article

Abstract: ASpectS Of geNe expReSSiON ANAlySiS iN SMAll-vOluMe blOOD SAMpleS uSiNg tHe ReAl-tiMe pcRThe gene expression profiling of peripheral blood leukocytes is a valuable method for assessing disease-associated and drug-response related genes.Small-volume blood samples can be used for gene expression analysis in biomarker disco very, pre-clinical animal research for drug development, neonatal and pediatric studies, and therapeutic drug monito ring.However, the reduced RNA quantity and handling procedures of small-volume samples complicate gene expression analysis using the quantitative real-time reverse transcription polymerase chain reaction.In this article, we systematized and compared the RNA isolation and molecular genetic techniques to disclose optimized strategies for the gene expression analysis of small-volume blood samples and discuss the gene expression studies in capillary blood samples.

Source: Biopolymers and Cell

DOI: 10.7124/bc.000b11

Authors: O. D. Hlazunova, N. S. Melnichuk

Institutions: Taras Shevchenko National University of Kyiv, Institute of Molecular Biology and Genetics

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W4409408181