Work

Novel Approach Using Real‐Time Dynamic Imaging Analysis to Monitor Cellular Apoptosis, Viability, and Cell Density in CHO Cell‐Based Bioprocesses for Monoclonal Antibody Production

Year: 2025

Type: article

Abstract: ABSTRACT We investigated an analytical method based on dynamic imaging analysis (DIA) for monitoring cellular apoptosis, viability, and cell density. Trypan blue and flow cytometry were used as reference methods. The DIA method showed results comparable to the traditional methods, with clear advantages (label‐free and in‐line real‐time monitoring capability). The DIA method successfully traced the trajectory of cell death progress, detecting the onset of apoptosis earlier than the other methods.

Source: Biotechnology Journal

DOI: 10.1002/biot.70018

Authors: Claudia Corredor, Yuxiang Zhao, Suyang Wu, James K. Drennen, Carl Anderson

Institutions: Bristol-Myers Squibb (United States), Duquesne University

Language: English

Topic: Viral Infectious Diseases and Gene Expression in Insects

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Syracuse University

OpenAlex ID: W4409408607

FWCI: 1.768

Cites: 31

Cited by: 7

Related to: 10