Dendritic Gold Nanoparticles Loaded on 3D Graphene-like Surface and Layer-by-Layer Assembly for Enhanced Glucose Biosensing
Year: 2025
Type: article
Abstract: Background/Objectives: In this study, AuDNs/EPLE composite electrodes with hierarchical dendritic nanogold structures were fabricated using the in situ electrodeposition of gold nanoparticles through the i-t method. Methods: A conductive polymer composite membrane, PEDOT, was synthesized via the electropolymerization of EDOT and the negatively charged PSS−. The negatively charged SO3− groups on the surface of the PEDOT membrane were electrostatically adsorbed with the glucose oxidase (GOD) enzyme and a positively charged chitosan co-solution (GOD/chit+). Using a layer-by-layer self-assembly approach, GOD was incorporated into the multilayers of the composite electrode to create the composite GOD/chit+/PEDOT/AuDNs/EPLE. Results: Electrochemical analysis revealed a GOD surface coverage of 8.5 × 10−10 mol cm−2 and an electron transfer rate of 1.394 ± 0.02 s−1. The composite electrode exhibited a linear response to glucose in the concentration range of 6.923 × 10−2 mM to 1.54 mM, with an apparent Michaelis constant of 0.352 ± 0.02 mM. Furthermore, the GOD/chit+/PEDOT/AuDNs/EPLE also showed good accuracy of glucose determination in human serum samples. Conclusions: These findings highlight the potential of the GOD/chit+/PEDOT/AuDNs/EPLE composite electrode in the development of efficient enzymatic biofuel cells for glucose sensing and energy harvesting applications.
Source: Biosensors
DOI: 10.3390/bios15040246
Authors: Zifeng Zhu, Yiming Zhao, Yongming Ruan, Xuexiang Weng, Gesmi Milcovich
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,654
OpenAlex ID: W4409409134