Work

Evaluation of TP53 Expression in Pediatric B-cell Acute Lymphoblastic Leukemia and Its Correlation With Clinicopathological Profiles

Year: 2025

Type: article

Abstract: Introduction: TP53 is a tumor suppressor gene which impacts tumor progression and prognosis in various cancers.TP53 isoforms regulate key processes like cell cycle arrest and apoptosis.Elevated TP53 expression in B-cell ALL correlates with poor outcomes, making it a potential prognostic marker and therapeutic target.This study aimed to evaluate the expression of TP53 isoforms and its correlation with clinicopathological profiles in pediatric B-cell ALL patients.Methodology: One hundred pediatric B-cell ALL patients (≤12 years) and 20 age matched healthy controls were enrolled for this study.Expression of TP53 full-length and isoform Delta40TP53, Delta133TP53, TP53 Beta expressions was checked using qRT-PCR.Fold change was calculated using 2-Ct method.T expression of isoforms was correlated with various clinical parameters such as risk stratification, blast count, molecular cytogenetics etc.Results: The median age of the patients was 5 years with male: female ratio of 1.2:1.52% patients had high expression of TP53 full gene expression with the maximum fold change of 9.99-fold.Isoform Delta40TP53, Delta133TP53 and TP53Beta were overexpressed in 17%, 53% and 6% of the patients respectively.

Source: OncoDaily Medical Journal

DOI: 10.69690/odmj-018-0425-1851

Authors: Hasib Tk, Parminder Kaur, Prateek Bhatia, Amita Trehan, Minu Singh

Institution: Post Graduate Institute of Medical Education and Research

Language: English

Topic: Acute Lymphoblastic Leukemia research

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W4409412124