3D-printed GelMA/SA/CMCS hydrogel scaffolds containing Cynomorium songaricum polysaccharide for critical bone defect repair
Year: 2025
Type: article
Abstract: Critical bone defect repair remains a major challenge in orthopedics. Cynomorium songaricum polysaccharide (CSP), derived from the traditional medicinal plant Cynomorium songaricum Rupr. in China, demonstrates excellent anti-inflammatory and osteogenic properties. Given these promising biological activities, we developed a novel therapeutic approach using a hydrogel composite scaffold incorporating CSP (GAC-C) for treating critical-sized bone defects. The composite scaffold was fabricated by embedding CSP into a methacrylated gelatin (GelMA)/sodium alginate (SA)/carboxymethyl chitosan (CMCS) blend via three-dimensional (3D) printing technology. The structural, mechanical, and biological properties of GAC-C were characterized, and osteogenic performance was evaluated both in vitro with rat bone marrow stromal cells (rBMSCs) and in vivo using a critical-sized bone defect model. Results indicated that the GAC-C scaffold demonstrated excellent biocompatibility, promoted osteogenic differentiation of rBMSCs, and enhanced bone integration and repair. Thus, the GAC-C scaffold has the potential for effectively repairing criticalsized bone defects.
DOI: 10.36922/ijb.8557
Authors: Dongdong Li, Chengxin Ruan, Zhiyuan Luo, Jiale Jin, Dongyu Wang, Yiqi Yang, Shenghu Zhou, Shuai Li, Pengfei Lei
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4409420839