Endophytic Bacteria as Biocontrol Agent Against Rigidoporus Microporus on Myristica Fragrans Houtt. Plant In Vitro
Year: 2025
Type: conference-paper
Abstract: Aceh Province, specifically South Aceh District, is one of the largest nutmeg producers in Indonesia. However, nutmeg production has decreased by 70%, due to the white root fungus attack caused by Rigidoporus microporus. The alternative control needs to be carried, by utilizing biological agents such as endophytic bacteria, which are able to produce secondary metabolites, enzymes, antibiotics, which are the mechanism to protect plants from pathogens. The research consisted of 15 endophytic bacterial isolates with 5 replicates. The stages of this research were exploration, isolation, purification, bacterial gram staining, isolation and purification of white root rot fungus, and inhibition test. The results showed that 15 endophytic bacteria isolates were successfully isolated and purified, which characterized as microscopic (9 isolates had coccus cell form and 4 isolates had bacilli cell form with negative gram, and 2 isolates had bacilli cell form with the positive gram), and various microscopic characteristics (size, colony shape, elevation and margin). The inhibition test showed that there were two isolates EB06 and EB15 act as biocontrol agents that had the highest percentage of pathogen inhibition (58.63% and 59.76%) and the strong inhibition categorize, also had the lowest white root fungus diameter i.e. 1.00 cm.
Authors: V Maulidia, A Agustinur, G Novia
Institution: Universitas Teuku Umar
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W4409429658