ARAP1‐AS1 Overexpression Increases Diffuse Large B Cell Lymphoma Progression by Sponging miR‐508–5p to Activate the EMP1‐PI3K/AKT Pathway
Year: 2025
Type: article
Abstract: Diffuse large B cell lymphoma (DLBCL) is a type of common and fatal non-Hodgkin lymphoma. This study aimed to detect the specific function of lncRNA ARAP1-AS1 in DLBCL. The in vitro experiments were performed using RT-qPCR, western blotting, MTT, colony formation, flow cytometry analysis, FISH, RNA pulldown, and luciferase reporter assays. A xenograft mouse model was used to evaluate tumor growth in vivo. ARAP1-AS1 expression was upregulated in DLBCL tissues and cells. ARAP1-AS1 knockdown inhibited DLBCL cell proliferation and promoted apoptosis. ARAP1-AS1 activated PI3K/AKT signaling by upregulating EMP1 expression via miR-508-5p. EMP1 overexpression markedly abolished the effect of ARAP1-AS1 knockdown on DLBCL cell proliferation and apoptosis, and PI3K inhibitor reversed the effect of ARAP1-AS1 overexpression on DLBCL cells. ARAP1-AS1 knockdown inhibited DLBCL tumor growth and reduced Ki-67, EMP1, and p-AKT expression in xenograft mouse models. ARAP1-AS1 knockdown exerts anti-tumor effect on DLBCL progression through the miR-508-5p/EMP1/PI3K/AKT axis.
Source: Hematological Oncology
DOI: 10.1002/hon.70056
Authors: Yamei Chen, Minmin Ren, Lei Zhu, Ting Sun, Xiaoyong Wang, Lingling Jiang, Linlin Zhou, Dongyun Gao
Institution: Nantong University
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4409431572