Work

Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution: ROA and CPL study

Year: 2025

Type: article

Abstract: Chiroptical studies of the SpyCas9 protein are extremely rare. Nondestructive methods are needed to characterize its active ribonucleoprotein form. Using Raman optical activity (ROA) and circularly polarized luminescence (CPL), we present a new approach to detect key biomolecules involved in CRISPR-Cas technology while preserving their original nucleolytic activity.

Source: Chemical Communications

DOI: 10.1039/d5cc00971e