Work

Simultaneous Expression of Recombinant Cellulase and Protease in An Indigenous Bacillus Cereus Strain.

Year: 2024

Type: article

Abstract: Background: Gene manipulation has a wide array of applications in microorganisms. We can construct multifunctional bacterial strains by gene manipulation and gene editing in order to produce several industrial biomaterials including enzymes at the same time. Objective: EG296 strain through gene manipulation. Materials and Methods: gene) was also deleted from the genome of the transformant by homologous recombination in order to increase the cellulase and protease activities simultaneously. Results: have an instability index of 26.16 and 20.18 respectively, which is much lower than threshold of 40. Accordingly, it can be concluded that both enzymes are considered to be stable. Conclusion: As a result, we obtained a genetically engineered strain that had the ability to produce and secrete two important industrial extracellular enzymes (cellulase and protease), with easy downstream purification processes.

Source: PubMed

DOI: 10.30498/ijb.2024.427242.3840

Authors: Zainab Farhangi, Farideh Tabatabaee Yazdi, Bagher Yakhchali

Institutions: Ferdowsi University of Mashhad, National Institute of Genetic Engineering and Biotechnology

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Ferdowsi University of Mashhad, National Institute for Genetic Engineering and Biotechnology

OpenAlex ID: W4409445051