Mutational Analysis of Colistin-Resistant Pseudomonas aeruginosa Isolates: From Genomic Background to Antibiotic Resistance
Year: 2025
Type: article
Abstract: This study analyzed eleven isolates of colistin-resistant Pseudomonas aeruginosa, originating from Portugal and Taiwan, which are associated with various pathologies. The results revealed significant genetic diversity among the isolates, with each exhibiting a distinct genetic profile. A prevalence of sequence type ST235 was observed, characterizing it as a high-risk clone, and serotyping indicated a predominance of type O11, associated with chronic respiratory infections in cystic fibrosis (CF) patients. The phylogenetic analysis demonstrated genetic diversity among the isolates, with distinct clades and complex evolutionary relationships. Additionally, transposable elements such as Tn3 and IS6 were identified in all isolates, highlighting their importance in the mobility of antibiotic resistance genes. An analysis of antimicrobial resistance profiles revealed pan-drug resistance in all isolates, with a high prevalence of genes conferring resistance to β-lactams and aminoglycosides. Furthermore, additional analyses revealed mutations in regulatory networks and specific loci previously implicated in colistin resistance, such as pmrA, cprS, phoO, and others, suggesting a possible contribution to the observed resistant phenotype. This study has a strong impact because it not only reveals the genetic diversity and resistance mechanisms in P. aeruginosa but also identifies mutations in regulatory genes associated with colistin resistance.
Source: Pathogens
Institutions: University of Trás-os-Montes and Alto Douro, LAQV Requimte, Universidade Nova de Lisboa, National Tsing Hua University, Chang Gung Memorial Hospital, Linkou Chang Gung Memorial Hospital, Universidade do Porto, National Institute of Health Dr. Ricardo Jorge, Centro de Ciência Animal e Veterinária, Inserm, Université Clermont Auvergne, Centre Hospitalier Universitaire de Clermont-Ferrand, Institut National de la Recherche Agronomique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Microbiologie Environnement Digestif Santé
Language: English
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,654
Awards: G1240268737, G138060719, G1509028180, G1522114529, G1977608718, G2012127377, G2339299466, G2346795057, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos, G2756637201, G2814222956, G3101299970, G332082475, Laboratório Associado para Ciência Animal e Veterinária, G4046065984, Epidemiology of colistin-resistant Pseudomonas aeruginosa from humans, animals and ecosystems: a one health approach using omics tools (OMIC4COLPA), G567200461, G5832795443, G6113984419, G6119126181, G6412077064, G6463117426, G6625491009, G6677885567, G6997658380, G824076568, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos, G8963217260
OpenAlex ID: W4409446823