Year: 2025
Type: article
Abstract: Enzyme immobilization, a critical strategy in biocatalysis, offers substantial improvements over soluble enzyme systems, particularly in industrial and dairy biotechnology. This review articulates the core concepts of enzyme immobilization, elucidating the benefits of augmented stability, enhanced reusability, and improved operational efficiency. A systematic analysis of diverse immobilization techniques, encompassing adsorption, entrapment, encapsulation, covalent conjugation, and cross-linking, is presented, with a focus on their respective merits and limitations. The application of immobilized enzymes in dairy processes, such as lactose hydrolysis and cheese maturation, and in broader industrial contexts, including biofuel synthesis and wastewater remediation, is critically examined. Furthermore, this review explores contemporary approaches to enhance enzyme stability and reusability, including the utilization of nanomaterials, biomimetic matrices, and genetic modification. These advancements aim to mitigate challenges associated with enzyme leaching, thermal denaturation, and economic viability, thereby facilitating the development of sustainable and cost-effective bioprocesses.
Authors: Khushi Jani, Ashok Thalkar
Institution: Parul University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W4409448011