Work

Penicillin-like mimotopes from autodisplayed Fv-antibody library inhibiting β-lactamase activity

Year: 2025

Type: article

Abstract: ) of the expressed Fv-antibodies and synthesized peptides was estimated using SPR measurements. The β-lactamase inhibition activity of the Fv-antibodies and synthetic peptides was estimated using colorimetry based on the formation of penicilloic acid. The penicillin-like mimotopes of the expressed Fv-antibodies and synthesized peptides were demonstrated to have β-lactamase inhibition activity in the bacterial lysates. Finally, the docking analysis of β-lactamase and the screened CDR3 sequences demonstrated that the screened CDR3 sequences were specifically bound to the binding sites of β-lactamase.

Source: Journal of Materials Chemistry B

DOI: 10.1039/d4tb02793k