Diagnostic Accuracy of Adenosine Deaminase Levels in Peripheral Blood Lymphocyte of Patients with Pulmonary Tuberculosis
Year: 2025
Type: article
Study design: Observational Study
Abstract: CONTEXT: Tuberculosis (TB) is a well-known global health problem with one-fifth of the global burden in India. Although there are diagnostic and prognostic markers for TB, there is lack of specificity and sensitivity of diagnostic methods. AIMS: The goal of this study was to assess the diagnostic value of blood lymphocyte adenosine deaminase (ADA) levels in pulmonary TB patients. SUBJECTS AND METHODS: This was a prospective observational case-control study. A total of 80 subjects were included in this study and were divided into two groups: 40 people who were healthy in every respect and 40 patients with pulmonary TB. ADA activity was measured in each group by the Giusti and Galanti method using a commercial kit. STATISTICAL ANALYSIS USED: Data were analyzed using t -test with receiver operating characteristic curve analysis done to identify the cutoff values for ADA that had maximum sensitivity and specificity. RESULTS: The mean serum ADA level in the pulmonary TB group was significantly increased as compared to control group ( P < 0.0001), whereas blood lymphocyte ADA levels as well as blood lymphocyte/serum ADA ratio in the pulmonary TB group were significantly decreased as compared control group ( P < 0.0001). In regard to the cutoff value of <34.06 IU/mg protein in patients with pulmonary TB, sensitivity and specificity were 90.00% and 80.00%, respectively. CONCLUSIONS: Blood lymphocyte ADA level may be used as a biomarker for the diagnosis of pulmonary TB.
Source: Annals of African Medicine
Institution: All India Institute of Medical Sciences Bhopal
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4409469817