Work

Evaluating the stability of Tritrichomonas foetus in various media under simulated environmental conditions of shipment for RT-qPCR assays

Year: 2025

Type: article

Abstract: Tritrichomonas foetus is a notable reproductive pathogen in cattle, and the processes of sample collection, handling, transportation, and testing present considerable challenges for surveillance initiatives. Advancements include the development of a direct detection method for T. foetus using reverse-transcription quantitative real-time PCR. We compared the stability of samples in 3 different media: PBS, sterile saline, and lactated Ringer solution over periods of 24–120 h, with storage temperatures of 0°C, 20°C, 37°C, and 54.5°C to mimic shipping of samples in various environmental temperatures.

Source: Journal of Veterinary Diagnostic Investigation

DOI: 10.1177/10406387251330297

Authors: SaraBeth Boggan, Ryan B. Williams, Jennifer H. Koziol

Institution: Texas Tech University

Language: English

Topic: Parasitic Infections and Diagnostics

Subfield: Parasitology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: green

Funder: American Association of Bovine Practitioners