Dual Immunization with Lipoprotein Tp0663 and Flagellin FlaB3 Offers Augmented Protection against Treponema pallidum in Mice
Year: 2025
Type: article
Abstract: High Resolution Image Download MS PowerPoint Slide Syphilis is a significant multistage sexually transmitted infection caused by the bacterium Treponema pallidum . The pathogenesis of this pathogen remains inadequately understood, impeding the progress of syphilis vaccine development. Our prior study has demonstrated the potential of the Tp0663 protein as a viable candidate for a vaccine against T. pallidum . In the present study, we sought to explore the protective response of dual immunization using two different antigenic entities (i.e., flagellin FlaB3 and lipoprotein Tp0663) against T. pallidum in a murine model. Our investigation revealed that FlaB3 + Tp0663 can elicit robust humoral and cellular immune responses. In addition, the FlaB3 + Tp0663 vaccine demonstrated a notable reduction in the Treponemal burden within various anatomical sites of infected mice, including the blood, brain, liver, lymph nodes, spleen, and testicles. It is worth noting that the FlaB3 + Tp0663 vaccine suppressed the dissemination of T. pallidum in C57BL/6 mice. The findings demonstrate that T. pallidum flagellin FlaB3 may augment the immunoprotection of Tp0663. This represents a valuable practical perspective and offers insights into developing a syphilis vaccine.
Source: ACS Omega
Authors: Yafeng Xie, Yiming Zhou, Jing Yang, Man Xu
Institution: University of South China
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,935
OpenAlex ID: W4409496980