Cover Feature: Boronic Acid‐Linked Apo‐Zinc Finger Protein for Ubiquitin Delivery in Live Cells (ChemBioChem 7/2025)
Year: 2025
Type: article
Abstract: Benzoboroxole facilitates the delivery of ubiquitin to living cells by utilizing the apo-zinc finger protein domain and thereby converts the apo-zinc finger protein (apo-ZFP) into a cell-penetrating peptide (CPP), despite being inherently a non-CPP. The introduction of point mutations within the skeletal structure, along with the incorporation of benzoboroxole into the lysine side chain, further enhances the efficacy of apo-ZFP. Cellular delivery was monitored using fluorescence microscopy and validated through flow cytometry. More details can be found inarticle 10.1002/cbic.202401040 by Pritam Ghosh and Oliver Seitz. Parts of the image were created with BioRender.com.
Source: ChemBioChem
Authors: Pritam Ghosh, Oliver Seitz
Institution: Humboldt-Universität zu Berlin
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4409502874