Work

Effects of Spent Mushroom Substrate Treated with Plant Growth-Promoting Rhizobacteria on Blueberry Growth and Soil Quality

Year: 2025

Type: article

Abstract: Spent mushroom substrate (SMS) is the residual biomass generated after harvesting the fruitbodies of edible fungi. It is produced in large quantities and contains abundant nutrients. Plant growth-promoting rhizobacteria (PGPR) are a group of plant-associated microorganisms known for their ability to enhance plant growth, improve disease resistance, and boost soil quality. In this study, three PGPR strains with the highest plant growth-promoting potential were selected based on their ability to grow effectively in SMS extract. The SMS substrates were mixed with PGPR solutions and sterile water to establish a batch culture system. The mixture was initially incubated at 28 °C for 3 days, followed by continuous aerobic decomposition in a ventilated environment for 180 days. Based on the quality analysis of the PGPR-treated SMS, the 54-day treatment for transplanting blueberry seedlings was selected. The PGPR-treated substrates showed significantly higher TN, HN, and AP than controls (p < 0.05), suggesting a potential role of PGPR in enhancing nutrient availability. Alpha diversity index analysis revealed significant differences in microbial diversity between the PGPR-treated substrates and the control. Furthermore, the PGPR-treated substrates significantly influenced plant growth characteristics, soil nutrient content, and rhizosphere microbial diversity. Enhanced plant growth characteristics were strongly correlated with increased soil nutrient levels, suggesting a potential link between rhizospheric microbial communities and plant growth performance. This study provides a novel approach and experimental framework for the utilization of SMS and the development of PGPR-based biofertilizers, offering valuable insights into sustainable agricultural practices.

Source: Microorganisms

DOI: 10.3390/microorganisms13040932

Authors: Mengjiao Wang, Desheng Sun, Zhimin Xu

Institutions: Shaanxi University of Technology, Louisiana State University

Language: English

Topic: Agriculture, Plant Science, Crop Management

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $3,258