Ketone body-supported respiration in murine isolated brain mitochondria is augmented by alpha-ketoglutarate and is optimized by neuronal SCOT expression
Year: 2025
Type: article
Abstract: This paper established a protocol to maximize ketone body respiration in isolated brain mitochondria while quantifying acetyl-CoA and energy charge in control mice compared with mice with neuron-specific deletion of succinyl-CoA-3-oxoacid-CoA transferase (SCOT) enzyme, required for ketone body oxidation. Findings are that alpha-ketoglutarate substantially increased acetoacetate (AcAc)-dependent respiration and neuron SCOT-KO had lower AcAc state 3 respiration with a decreased acetyl-CoA level.
Language: English
Topic: Diet and metabolism studies
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Funders: National Institutes of Health, Centers for Disease Control and Prevention, National Institute on Aging, National Heart, Lung, and Blood Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, Division of Diabetes, Endocrinology, and Metabolic Diseases
Awards: G1271631115, G1893859378, G2293305041, Metabolic Phenotyping Cluster, G5032276587, G5272479806, G535324671, G5844433898, G5925985626, G5936157174, Hepatic mitochondrial function control of high-fat diet-induced weight gain, Inclusive Excellence Training Program in the Systems Biology of Cardiovascular Inflammation
OpenAlex ID: W4409537895