Work

Microstructural imaging of the cartilaginous endplate in the intervertebral disc

Year: 2025

Type: article

Abstract: 1 Objective: Intervertebral disc (IVD) degeneration is a major risk factor for the development of chronic low back pain (LBP), affecting millions globally, particularly as they age. IVD degeneration can promote inflammation, and reduce spinal stability leading to compression of nerve roots. Research has primarily focused on the annulus fibrosus (AF) or nucleus pulposus (NP), with an unmet need to explore the physiology of the cartilaginous endplate (CEP) and its microstructural assessment in IVD degeneration. Imaging the microstructure of the CEP, a relatively thin layer between the IVD and vertebral body, is challenging. This mini-review discusses advanced imaging methods to capture the microstructure of the CEP. 2 Design: microstructural imaging of the CEP using advanced techniques such as three-dimensional (3D) micro-computed tomography (µCT) and two-dimensional (2D) light microscopy. 3 Results: Advanced imaging techniques can provide detailed visualization of IVD microarchitecture, structural integrity, and degeneration, including CEP structure. Optical light microscopy techniques provide detailed 2D images of the CEP from stained or unstained IVD sections. Imaging the IVD in a 3D modality, such as contrast-enhanced µCT, enables a better understanding of the CEP's microstructural changes in IVD degeneration. 4 Conclusions: . Moreover, analyzing CEP-driven microstructural degeneration is crucial for developing preclinical anatomical models that spine surgeons can use to test and evaluate medical and therapeutic candidates.

Source: Osteoarthritis Imaging

DOI: 10.1016/j.ostima.2025.100269

Authors: Valtteri Peitso, Shuvashis Das Gupta, Ali Mobasheri

Institutions: University of Oulu, University of Liège, State Research Institute Centre for Innovative Medicine, The First Affiliated Hospital, Sun Yat-sen University

Language: English

Topic: Spine and Intervertebral Disc Pathology

Subfield: Pathology and Forensic Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $1,400