Adoption of a novel medium for the industrial (3000 L) production of Serendipita indica employing a nutrient limitation strategy using insoluble carbon and phosphate sources
Year: 2024
Type: article
Abstract: The use of the endophytic fungus Serendipita indica has rapidly increased due to its wide range of host species, ability to foster plant-growth, and ability to confer tolerance to a number of stresses. However, its industrial-scale production is still in its infancy due to its low-biomass yield and prolonged cultivation time. Thus far, Hill-Kafer medium has traditionally been used for S. indica cultivation, resulting in lower yields and excessively long incubation times. Here, we adopted a simple insoluble carbon and phosphate input medium for rapidly generating high biomass. We developed and optimized the SIF1 medium, achieving maximum biomass production (424.5 ± 1.9 g/L), significantly outperforming Hill-Kafer medium. Statistical optimization of SIF1 identified optimal levels (15 g/L oats, 7.5 g/L tricalcium phosphate, 95-hr incubation). Validated results in the laboratory (FUS-10 L: 484.4 ± 4.7), pilot (300 L: 496.5 ± 7 g/L), and industrial (3000L: 492.4 ± 7.1 g/L) bioreactors proved the efficacy of SIF1. Compared to Hill-Kafer (54.8 ± 3.7 g/L), SIF1 showed nine-fold higher biomass productivity and reduced cultivation time by approximately 6 days. Based on our findings, it appears that SF1 will be a highly efficient medium for producing S. indica on an industrial scale and expanding its use. ONE-SENTENCE SUMMARY: This study presents a rapid industrial production strategy for the beneficial fungus Serendipita indica, providing a scalable solution for wider applications and contributing to global food security and environmental sustainability.
DOI: 10.1093/jimb/kuaf009
Language: English
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,067
OpenAlex ID: W4409544034