Co-stimulation with equibiaxial strain and pre-osteoblast co-culture differentiates monocytes in a bone model
Year: 2025
Type: article
Abstract: Bone remodeling and immune function are dynamically regulated through cell-cell and cell-matrix interactions by stem and mature cell populations. We investigated the hypothesis that monocytes and pre-osteoblasts respond to cyclic tensile stress and paracrine interactions by differentiating into macrophage-like and osteoblast-like cells. 20% cyclic equibiaxial strain was applied to monocytic U937 and pre-osteoblastic ST2 cells for 72 h. Increased levels of CD11B, CD14, IL-6, and IL-8 in U937 indicated monocytic differentiation. Increased ALP expression and calcium deposition in ST2 indicated differentiation towards osteoblastic lineage. Overall, application of cyclic strain and pre-osteoblastic co-culture induced differentiation in this cyclically strained bone model.
Source: MRS Communications
Authors: Maria R. Ward Rashidi, Catherine S. Snyder, Kathleen M. Burkhard, Raneem Ahmad, Isha Bhorkar, Geeta Mehta
Institutions: University of Michigan, U-M Rogel Cancer Center
Language: English
Topic: Bone Metabolism and Diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,390
Awards: G1404790370, G1519688000, Ovarian Cancer Tumoroids to Study Heterogeneity and Chemoresistance, EFRI DCheM: Distributed Manufacturing of Personalized Medicines, G3398315005, IDENTIFICATION OF DISTINCTLY EXPRESSED GENES AND ALTERED METABOLIC PATHWAYS IN A PHYSIOLOGICALLY RELEVANT 3D SPHEROID MODEL OF OVARIAN CANCER, G4119534169, HIGH-THROUGHPUT PLATFORM FOR PATIENT-DERIVED, SMALL CELL NUMBER, THREE-DIMENSIONAL OVARIAN CANCER SPHEROIDS, G496050054, G509143833, G8166743225, G8632625050, University of Michigan Rogel Cancer Center Support Grant 2023-2028
OpenAlex ID: W4409552492