Natural Killer Cell Membrane Isolation with Minimal Nuclear, Mitochondrial, and Cytosol Contamination
Year: 2025
Type: article
Abstract: Cell membrane isolation is essential for diverse biological investigations, ranging from fundamental research to advanced therapeutic applications. This study compared two methods-differential centrifugation and discontinuous sucrose density gradient ultracentrifugation-for isolating cell membranes from the human natural killer (NK) cell line (KHYG-1). The aim was to identify the method that minimizes contamination from nuclear, mitochondrial, and cytosolic components. Differential centrifugation yielded approximately 8 mg of cell membrane, whereas sucrose density gradient ultracentrifugation produced about 5 mg. Despite the lower yield, the latter method exhibited superior performance due to significantly reduced contamination. This protocol is adaptable to various cell types, offering a reliable approach for producing cell membrane-coated mimics for therapeutic use. The increasing demand for isolated cell membranes in biomedical applications highlights the importance of optimizing isolation techniques.
Authors: Vaishali Chugh, Vijaya Krishna K., Vaibhav Patil, Abhay Pandit
Institution: Ollscoil na Gaillimhe – University of Galway
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W4409570412