Comparative HPLC-DAD-ESI-QTOF-HR-MS/MS analyses of phenolic compounds of authentic lemon, lime, orange, and grapefruit juices
Year: 2025
Type: article
Abstract: Detailed knowledge about phenolic compounds of Citrus fruits and derived juices is important for investigations into Citrus product authentication as well as for their potential health benefits. We present an HPLC-DAD-ESI-QTOF-HR-MS/MS method for the identification and simultaneous quantitation of more than 50 compounds ranging from polar phenolic acids over flavonoid glycosides to apolar coumarins, psoralens, and polymethoxyflavones in lemon, lime, orange, and grapefruit juices. Hydroxycinnamic acid derivatives mainly comprised esters with aldaric acids, N - p -coumaroylputrescine, and N -feruloylputrescine. Predominant flavonoids were flavone- C - and O -glycosides, flavonol- and flavanone- O -glycosides, and polymethoxyflavones. Mass fragmentations of flavone- C - and O -glycosides as well as limocitrin glycosides and their acylated derivatives carrying malonyl- and/or hydroxymethylglutaryl moieties are proposed. The presence of diverse phenolic compounds in juices from four Citrus species was confirmed and numerous novel constituents were assigned. Total levels of phenolic constituents in the aforementioned Citrus juices are presented. • Comparative analysis of authentic lemon, lime, orange and grapefruit juices • Identification and quantitation of more than 50 phenolic compounds in the juices • Structural assignment of novel Citrus constituents by ESI-QTOF-HR-MS/MS • Juices contained hydroxycinnamic acids, flavonoids, coumarins, and psoralens • Reveals contribution of the individual classes on total phenolic compounds
Source: Food Chemistry
Institutions: Hochschule Geisenheim University, SGF International (Germany)
Language: English
Subfield: Biochemistry
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,930
Funder: Deutsche Forschungsgemeinschaft
Award: G4875002709
OpenAlex ID: W4409571963