Comprehensive evaluation and application of tissue clearing techniques for 3-D visualization of splenic neural and immune architecture
Year: 2025
Type: article
Abstract: This study systematically assessed five tissue-clearing techniques and optimized the conditions of each protocol to overcome the challenges of splenic 3-D imaging posed by its dense structure and high pigmentation. The results demonstrated SHANEL and advanced CUBIC as the optimal methods for 3-D visualization of diverse splenic immune and neural architecture, with which we successfully mapped splenic neuroimmune landscape and identified nociceptive nerves within the human spleen for the first time.
Authors: Jianing Li, Letian He, Wenling Wang, Siyu Wang, Dan Zhang, Liyun Liang, Guangping Song, Yijian Zhang, Shaoqing Yu, Lei Wang, Qiuying Han, Shaoyi Huang, Sen Li, Haiqing Tu, Zengqing Song, Huaibin Hu, Huiyan Li, Yang Yang, Min Wu
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: bronze
Awards: G1685081438, G2839121085, G6207684680
OpenAlex ID: W4409574958