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Expression and Characterization of a Rice β-Xylosidase with Xylooligosaccharide Hydrolysis and Transglycosylation Activities

Year: 2025

Type: article

Abstract: High Resolution Image Download MS PowerPoint Slide Plant β-xylosidases are less well characterized for hemicellulose degradation than their microbial counterparts. To address this, a broadly expressed rice ( Oryza sativa ) glycoside hydrolase family 3 (GH3) β-xylosidase designated OsXyl1 was expressed in heterologous Pichia pastoris . OsXyl1 showed maximal enzyme activity at pH 4.0 and 60 °C. It was relatively stable at 30–50 °C. It hydrolyzed 4NP-β- d -xylopyranoside (4NPXyl) and β-1,4-linked xylooligosaccharides (XOS) with degrees of polymerization (DP) of 2–6. OsXyl1 hydrolylsis of 4NPXyl was much more rapid and specific than that of other 4NP glycosides with an apparent k cat / K m value of 19.0 mM –1 s –1 . OsXyl1 had similar specificity toward XOS having DP values of 2–5 with apparent k cat / K m values of 2.6–4.2 mM –1 s –1 . OsXyl1 was also efficient at transglycosylating short alcohols with 4NPXyl and XOS xylosyl donors. Therefore, rice OsXyl1 β-xylosidase may function in recycling of xylans in plant cell wall recycling and it may be applied for transglycosylation of alcohol acceptors.

Source: Journal of Agricultural and Food Chemistry

DOI: 10.1021/acs.jafc.4c13281

Authors: Kadsada Sala, Salila Pengthaisong, Chamaipon Beagbandee, James R. Ketudat Cairns

Institution: Suranaree University of Technology

Language: English

Topic: Polysaccharides and Plant Cell Walls

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,500

Funders: Suranaree University of Technology, Thailand Science Research and Innovation