Work

Acetylated glucomanno-oligosaccharides from human gut microbial degradation of Dendrobium polysaccharides: production and effects on anti-hyperglycemia-related factors

Year: 2025

Type: article

Abstract: Dysbiosis of gut microbiota is tight related to metabolic diseases, and are affected by several factors, and the indigestible carbohydrates from food and herbal medicine that are predominantly metabolized and utilized by gut microbes are thought to play key roles. Dendrobium is a noble traditional Chinese herb medicine rich in glucomannan with bioactivities such as regulating blood sugar. Dendrobium Taiseed Tosnobile (DTT) is a potential Dendrobium variety. In vivo studies suggested that the polysaccharides of DTT (DTT PS ) demonstrated significant activities in regulating hyperglycemia-related physiological conditions and increased the abundance of probiotics. Via chemical and spectroscopic strategies, the main polysaccharide of DTT PS was confirmed to be a partially acetylated glucomannan with a Mn of 194 kDa. To verify whether the indigestible carbohydrates of the DTT PS regulate blood glucose level, a glucomannan-degrading microbe ( Bacteroides ovatus ) was chosen to ferment DTT PS . DTT PS F2, an acetylated glucomanno-oligosaccharide-rich fraction with a MW range of 0.5–2 kDa, was successfully obtained. The molecular docking results suggested the acetyl substituents of DTT PS effectively hinder the binding with Bo GmuGs ( endo -1,4-β-mannosidases of B. ovatus ), thereby limiting the degradation. These oligosaccharides exhibited potent activities in promoting GLP-1 accumulation. These findings revealed its potential role of DTT PS F2 in regulating hyperglycemia.

Source: Carbohydrate Polymer Technologies and Applications

DOI: 10.1016/j.carpta.2025.100808