Genotypes of Blastocystis Hominis Isolated From Iraqi Patients With Irritable Bowel Syndrome
Year: 2024
Type: article
Study design: Observational Study
Abstract: From October 2022 to February 2023, a total of 172 stool samples were collected from patients and healthy control participants attending the Gastroenterology and Endoscopy Unit at the Digestive System Medical City/Baghdad Hospital.Sixty eight individuals with irritable bowel syndrome (IBS) were found to have Blastocystis hominis (39.5 percent).Because the clinical complaint with the greatest rate (57.3 percent) was stomach discomfort and flatulence, which was statistically highly significant (p0.01).39 out of 58.6 percent were statistically significant (p0.05).In comparison, the clinical symptom with the lowest rate (10.3 percent) was statistically insignificant (p0.05).B. hominis genotypes were identified using restriction fragment length polymorphism (RFLP) analysis of PCR amplified small subunit rDNA.The extraction of genomic DNA from 20 Blastocystis isolates was effective by using E.Z.N.A. stool extraction kit.For PCR amplification, three sets of sequence tagged site (STS) primers were used: SB 83, SB 227, and SB 155.Out of 66 (38.3 percent) positive Blastocystis samples, 10 (15.5 percent) were chosen at random for conventional PCR amplification.Subtypes 1, 3, and 7 were the ones that were amplified.Subtype 1 had the greatest rate (7.3 percent), followed by subtype 7 (7.3 percent), and subtype 3 had the lowest incidence (4.4 percent ).The Nucleotide Sequence of B. hominis genes indicated that a mutation occurred in the area of (TC) in the position of (T601C) and that it was reversed from (ATA) to (ACA) with a forward primer.In conclusion, the clinical symptom with the highest prevalence was abdominal pain, which was followed by flatulence, and genotypes were classified into various subtypes: Subtype 1 had the highest incidence of occurrence.The primary aims of our study were to identify the main clinical symptoms in individuals with irritable bowel syndrome and B. hominis, as well as to identify the genotypes of B. hominis.
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4409604997