Work

Enzymatic hydrolysis of cottonseed protein enhances appetite and intestinal health of Litopenaeus vannamei

Year: 2025

Type: article

Abstract: This study examined the effects of enzymatic hydrolysis cottonseed protein (ECP) on the appetite and intestinal health of Litopenaeus vannamei (0.16 ± 0.01 g). Based on the control diet (fishmeal 18 %, crude protein 40 %, crude fat 7.5 %), 2 %, 4 %, 6 %, 8 % and 10 % of ECP were added to replace fishmeal in equal amounts to formulate six isonitrogenous and iso-lipidic experimental diets named ECP0 (Control), ECP2, ECP4, ECP6, ECP8, and ECP10, respectively. After an 8-week feeding trial, the serum orexin levels were significantly increased ( P < 0.05); and leptin, peptide YY and cholecystokinin were significantly reduced when shrimp ingested diet contained ECP， as well as their genes expression levels ( P < 0.05). Compared with the ECP0 group, the weight gain rate of the shrimps in group ECP2 had significantly increased ( P < 0.05). Besides, intestinal fold height and muscular thickness of shrimps in group ECP2 were markedly increased, and up-regulated genes expression levels for periplasmic membrane factors ( P < 0.05). Significantly increased intestinal amylase, lipase, trypsin and chymotrypsin activities were found in shrimps fed diet ECP2 and ECP4 ( P < 0.05). The addition of ECP significantly decreased Vibrio , Photobacterium and Tenacibaculum abundance, and increased Ruegeria and Marivita abundance in shrimp intestine ( P < 0.05), especially the supplemented 2 % ECP significantly improved the membrane transport and amino acid metabolism functions of the intestinal flora in shrimps ( P < 0.05). Therefore, the addition of 2–4 % ECP by replacing fishmeal at an equal level could enhance growth by improving appetite, intestinal morphology and the composition of gut microbiota for L.vannamei . • Optimal growth performance of Litopenaeus vannamei was achived when 2–4 % ECP replaced fishmeal in equal proportions. • It promotes up-regulation of appetitive factors and down-regulation of inhibitory factors of shrimp through ECP’s appetizing. • ECP has the property to improve intestinal development and the structure of intestinal flora.

Source: Aquaculture Reports

DOI: 10.1016/j.aqrep.2025.102809