Chronopharmacology of the fisetin/glimepiride combination in the amelioration of diabetic retinopathy in rat model
Year: 2025
Type: article
Abstract: Diabetic retinopathy is a complication of diabetes caused by high blood sugar levels that damage the back of the eye (retina), potentially leading to blindness. The aim of the present study is to investigate the efficacy of fisetin and glimepiride combination in the management of diabetic retinopathy through a chronopharmacological approach. For the in vivo chronopharmacological study, male Wistar rats were divided into six groups, each comprising six animals. The treatment groups received 100 mg/kg of fisetin and glimepiride at different circadian time points (8:00 h, 14:00 h, and 20:00 h) for 28 consecutive days. The blood glucose levels of the animals were monitored during the treatment. On day 29, rats were euthanized, and biochemical and histopathological analyses were performed using a section of the rat eye. The group that received fisetin and glimepiride at 20:00 h showed a significant reduction in the glucose levels (9.46±7.86 mmol/L, p<0.05), decreased cholesterol and triglyceride levels, as well as lower malondialdehyde level compared to other treated groups and the positive control group. It can be concluded that the administration of glimepiride with fisetin at 20:00 h was more effective than other groups and that this administration time will reduce diabetic retinopathy complications. However, further studies are needed to elucidate these effects before clinical translation for managing diabetic retinopathy.
Source: Biologica Nyssana
Authors: Arumugam Madeswaran, Velumani Mathivanan
Language: English
Subfield: Ophthalmology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4409632396