Work

Three-year QTL mapping discovers a novel locus and candidate gene BnC09.LMI1 regulating the leaf complexity of Brassica napus

Year: 2025

Type: article

Abstract: Leaf shape diversity in Brassica napus provides critical insights into the molecular basis of leaf complexity and its evolutionary implications in rapeseed. Two predominant leaf morphotypes were characterized in B. napus : serrated-margin and deeply lobed leaves. Serrated leaves were further subdivided based on the presence or absence of petiolar lobes, establishing a novel phenotypic classification system. Through QTL mapping of the APL01/Holly recombinant inbred line population ( n = 2,755 markers) across three environments, we identified 10 consensus loci associated with non-lobed petioles. A major-effect QTL ( qNLP.C9-3 ) spanning a 951-kb interval on chromosome C9 accounted for 9.43–21.66% of phenotypic variation in the APL01 genetic background. Within this interval, 148 annotated genes were identified, among which BnC09.LMI1 was prioritized as a candidate gene regulating petiole lobe formation and leaf complexity based on phylogenetic analysis and differential expression. These results advance our understanding of leaf morphological diversity in B. napus and provide a molecular framework for dissecting the genetic architecture of leaf complexity.

Source: Plant Growth Regulation

DOI: 10.1007/s10725-025-01323-5

Authors: Lei Cai, Qianhui Zhou, Ting Ding, Xufan Zhou, Entang Tian, Kunjiang Yu

Institution: Guizhou University

Language: English

Topic: Genetic Mapping and Diversity in Plants and Animals

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $3,690