CRISPR/dCas9-targeted H3K27me3 demethylation at the CUC3 boundary gene triggers ectopic transcription and impacts plant development
Year: 2025
Type: article
Abstract: locus induces ectopic transcription and gene expression patterns, leading to altered leaf morphology and meristem integrity. Combining molecular and phenotypic analyses, we thus establish evidence directly linking H3K27me3-mediated repression to developmental outcomes. Our study highlights locus-specific epigenetic editing as a powerful approach to dissect the functional impacts of histone modifications on transcription and morphogenesis, and provides a framework for unveiling the causal role of chromatin dynamics in plant developmental plasticity.
Source: iScience
Authors: Kateryna Fal, Salim El Khoury, Marie Le Masson, Alexandre Berr, Cristel C. Carles
Institutions: Centre National de la Recherche Scientifique, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, CEA Grenoble, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Université Grenoble Alpes, Institut de Biologie Moléculaire des Plantes, Université de Strasbourg
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,240
Funder: Agence Nationale de la Recherche
OpenAlex ID: W4409643643