Work

Antibacterial effects of rosella petal extract (Hibiscus sabdariffa L.) in mouthwash formulation against Streptococcus sanguinis and Porphyromonas gingivalis Biofilms: An in vitro study

Year: 2025

Type: article

Abstract: in global prevalence and is expected to rise. Periodontal disease, caused by bacterial plaque, is a leading cause of tooth loss, impacting mastication, aesthetics, and quality of life. Plaque, a biofilm of microorganisms, triggers a chronic inflammatory response leading to dysbiosis and clinical disease. Microorganisms like Streptococcus sanguinis and Porphyromonas gingivalis play a crucial role in forming periodontal biofilm, with virulence factors that damage tissues. While chlorhexidine gluconate is effective in removing biofilm, it has long-term side effects. Therefore, natural ingredient-based mouthwashes are being researched. Rosella flower petals (Hibiscus sabdariffa L.) have antibacterial properties inhibiting S. sanguinis and P. gingivalis. They also exhibit antimicrobial, antioxidant, and anti-inflammatory activities. Previous studies found Rosella extract effective in inhibiting biofilm formation of these bacteria compared to chlorhexidine, but its effects in mouthwash formulations are unstudied. The phytochemical, organoleptic, pH, viscosity, antibiofilm activity of various mouthwash formulations containing Rosella petals extract has been evaluated. Rosella extract showed significant antibiofilm activity against both bacteria. The formulations maintained acceptable organoleptic properties, stable pH levels, and consistent viscosity. Higher Rosella concentrations correlated with increased effectiveness. Rosella extract is a promising natural ingredient for mouthwash, providing effective therapeutic antibiofilm activity against periodontal pathogens better than chlorhexidine. Further research is recommended to optimize formulation and assess long-term benefits and safety.

Source: The Saudi Dental Journal

DOI: 10.1007/s44445-025-00007-0

Authors: Trijani Suwandi, Mikha Sundjojo, Caesary Cloudya Panjaitan, Evangelista Rachel Hepziba, Tirza Oktarina Setiabudi

Institution: Universitas Trisakti

Language: English

Topic: Oral microbiology and periodontitis research

Subfield: Periodontics

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,490