Work

Genome-wide identification and characterization caleosin genes in lima-bean (Phaseolus lunatus)

Year: 2025

Type: article

Abstract: Phaseolus lunatus, commonly known as lima bean or butter bean, is a leguminous crop with significant agricultural and nutritional value, particularly in tropical regions. Caleosin, a lipid-associated protein, plays a crucial role in seed germination, stress response, and lipid metabolism, presenting potential targets for genetic improvement. This study aimed to identify and characterize caleosin genes (PlCLOs) in P. lunatus using in silico methods. Six genes encoding caleosin proteins were identified, exhibiting molecular weights between 16.58 and 27.28 kDa and subcellular localization predominantly in chloroplasts. Conserved motifs, such as calcium-binding and phosphorylation sites, were identified, alongside structural elements crucial for lipid droplet anchoring. Phylogenetic analysis revealed three evolutionary groups, suggesting functional divergence. Structural modeling confirmed high-quality protein models dominated by α-helices and irregular loops. Functional annotations highlighted roles in stress tolerance, calcium signaling, and lipid metabolism. These findings deepen the understanding of caleosins role in plant biology, providing insights for sustainable agricultural practices and genetic improvement of P. lunatus.

Source: Scientific Electronic Archives

DOI: 10.36560/18320252068

Authors: Maria Cidinária Silva Alves, Ramon da Silva de Souza, Caio Gabriel Tavares Ferreira, Luana de Souza Leandro, Dalton Ferreira Matos, Ruana Carolina Cabral da Silva

Institution: Universidade Federal de Pernambuco

Language: English

Topic: Lipid metabolism and biosynthesis

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

Funders: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico