TEMI: tissue-expansion mass-spectrometry imaging
Year: 2025
Type: article
Abstract: The spatial distribution of diverse biomolecules in multicellular organisms is essential for their physiological functions. High-throughput in situ mapping of biomolecules is crucial for both basic and medical research, and requires high scanning speed, spatial resolution, and chemical sensitivity. Here we developed a tissue-expansion method compatible with matrix-assisted laser desorption/ionization mass-spectrometry imaging (TEMI). TEMI reaches single-cell spatial resolution without sacrificing voxel throughput and enables the profiling of hundreds of biomolecules, including lipids, metabolites, peptides (proteins), and N-glycans. Using TEMI, we mapped the spatial distribution of biomolecules across various mammalian tissues and uncovered metabolic heterogeneity in tumors. TEMI can be easily adapted and broadly applied in biological and medical research, to advance spatial multi-omics profiling.
Source: Nature Methods
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $12,690
Funders: Howard Hughes Medical Institute, U.S. Department of Health and Human Services, U.S. Department of Agriculture, University of Wisconsin-Madison, University of Wisconsin Carbone Cancer Center, National Institutes of Health, Centers for Disease Control and Prevention, National Institute on Aging, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Research Resources
Awards: Creating a region- specific biomolecular atlas of the brain of Alzheimer’s disease, G1200757997, G1359571829, Acquisition of a High-Field Dual Source FTICR-MS for Pharmaceutical Research, G1629463853, G2053725305, G2118924489, G2284066598, G2337220457, G2388130288, G3042713858, G3055837027, G3396811048, G38122780, G3863694299, G4115624301, G6019913678, DiLeu-enabled multiplexed quantitation for biomarker discovery and validation in Alzheimer’s disease, Mass Spectrometric Studies of Neuropeptides in Feeding, Acquisition of a High Resolution High Speed MALDI Mass Spectrometer for Biomedical Research at UW-Madison, Acquisition of a Dual-Source, High-Performance, Ion Mobility, Quadrupole Time-of-Flight Mass Spectrometry System for Biomedical Research at UW-Madison, G822836364, G9000025623, G9000041967, G9000071658, G9000127380, G9000131312, G9000153645, G9000197343, G9000214629, G9000231773, G9000322327
OpenAlex ID: W4409681913