Work

Analysis of Radiation-induced DNA Damages by Means of Quantitative Polymerase Chain Reaction

Year: 2025

Type: article

Abstract: We have developed the method to detect DNA damage within the polymerase chain reaction (PCR) amplification region and estimate the risk of secondary cancer induction in radiation cancer therapy. DNA samples were irradiated with γ-rays, and oxidative damage and abasic sites were converted into strand breaks using formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease (III), respectively. The yields of DNA damage were subsequently evaluated by PCR. The ratio of strand breaks : abasic sites : oxidative damage was approximately 1.0 : 2.8 : 2.4, revealing the proportions of different apparent DNA damage.

Source: RADIOISOTOPES

DOI: 10.3769/radioisotopes.740206

Authors: Miyabi Yanami, Youichirou Matuo, Kikuo Shimizu, Yoshinobu Izumi

Institution: University of Fukui

Language: English

Topic: Effects of Radiation Exposure

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Japan Society for the Promotion of Science