Year: 2023
Type: preprint
Abstract: Aqueous strategies for whole-brain delipidation involve lipid removal via phase separation and detergent washes. SBiP is an aqueous biphasic buffer that extracts lipids at polar-nonpolar solvent interfaces. The brain is then washed with the detergent-based B1n buffer, which further disrupts residual membranes, forming micelles that can be washed out. When paired with organic delipidation, these steps can return a solvent-shrunken brain to normal size in phosphate buffer, suitable for post-delipidation antibody labeling.
Authors: Andrew Recknagel, Kevin Cao, Naveen Ouellette, Molly Logsdon, Judith Baka, Jayaram Chandrashekar, Rajvi Javeri
Institution: Allen Institute for Neural Dynamics
Language: English
Subfield: Filtration and Separation
Field: Chemical Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W4409728063