Work

Analysis and prediction of immune cell infiltration characteristics in COPD: Folium isatidis and its active ingredients are able to combat lung lesions caused by COPD by correcting immune cell infiltration

Year: 2025

Type: article

Abstract: Objective: As a respiratory disease, chronic obstructive pulmonary disease (COPD) has become a common fatal disease worldwide. We aimed to screen active traditional Chinese medicines (TCMs) for the treatment of COPD by COPD-related transcriptome gene chip analysis and verify their therapeutic activity for COPD. Methods: We used bioinformatics analysis to predict potential therapeutic TCMs based on the immune cell infiltration characteristics in COPD. Further, BALB/c female mice were divided into different treatment groups to investigate the effect of indirubin (IDR) and FI on COPD. After 12 weeks of intranasal lipopolysaccharide instillation and passive smoking, we started giving drug treatment to mice, including distilled water (control and model groups), dexamethasone, IDR and FI. The lung function, visceral index, degree of lung tissue damage, and immune cell infiltration were assessed. Results: experimental results showed that IDR and FI had protective effects against lung injury in mice and could inhibit COPD. The mechanism of this effect may be related to their ability to regulate the proportion and distribution of immune infiltration of B lymphocytes, CD4+ and CD8+ T lymphocytes, Treg cells, NK cells, myeloid-derived suppressor cells, and eosinophils. Conclusion: IDR and FI can ameliorate disease development in COPD model mice by regulating immune cell infiltration. This offers an experimental groundwork for delving deeper into the mechanisms through which TCMs impact COPD treatment and for identifying possible therapeutic drugs for COPD.

Source: Frontiers in Medicine

DOI: 10.3389/fmed.2025.1584411

Authors: Faqin Wang, Weichen Zhang, Yujie Huang, Xianbang Hou, Liwei He, Huiqin Xu

Institutions: Nanjing University of Chinese Medicine, Nanjing University of Chinese Medicine Hanlin College

Language: English

Topic: Chronic Obstructive Pulmonary Disease (COPD) Research

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,801