Gingival Inflammation Modulates NLRP3 Inflammasome Signalling in Peripheral Blood Mononuclear Cells of PCOS Patients: A Case-Control Study
Year: 2025
Type: article
Study design: Observational Study
Abstract: Polycystic ovary syndrome (PCOS) is a complex condition associated with chronic inflammation and oxidative stress and is often linked to periodontal diseases. This study aimed to determine whether gingivitis modulates the NLRP3 inflammasome in peripheral blood mononuclear cells (PBMCs) from women with PCOS. Following a case-control design, 104 women were divided into three groups: controls (n = 36), PCOS without gingivitis (PCOS, n = 44) and PCOS with gingivitis (PCOS+, n = 24). Periodontal parameters, proinflammatory regulators (NFκB p65, JNK), NLRP3 components (NLRP3, ASC, procaspase-1, caspase-1) and oxidative stress markers (superoxide, NRF2, GCLC and GSR) were determined. The PCOS+ group presented elevated values for bleeding on probing (BOP) and plaque and calculus indices, both of which were associated with increased protein levels of NFκB p65 and JNK, thus indicating NLRP3 inflammasome priming. Higher protein levels of NLRP3, ASC, procaspase-1 and caspase-1 in the PCOS+ group confirmed that priming had occurred, suggesting an engagement in assembly. When potential assembly signals of inflammasome were evaluated, the patients with PCOS generally presented enhanced total superoxide and an impaired antioxidant response (NRF2, GCLC and GSR). Moreover, BOP was independently associated with JNK, ASC and procaspase-1. These findings suggest that gingival inflammation modulates the innate immune response in leukocytes of women with PCOS via the NLRP3 inflammasome pathway, which is regulated by proinflammatory factors and oxidative damage.
Source: Antioxidants
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,499
Funders: Ministerio de Ciencia, Innovación y Universidades, European Commission, Generalitat Valenciana, Universitat de València, Ministerio de Ciencia e Innovación, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana, HORIZON EUROPE Framework Programme, Instituto de Salud Carlos III, European Regional Development Fund
Awards: G1034023686, G1078701667, G1154069931, G1234911041, G1611690318, G1675758422, G1691553913, G1841662612, G2002996012, G2186515320, G226790397, Efecto del tratamiento periodontal no quirúrgico sobre la respuesta inmune innata y adquirida desde una perspectiva de género: Posibles implicaciones terapéuticas de los miRNAs, G3931688971, G3969722732, G425566829, G4424906695, G4472067862, G4556839000, G461227938, G4687378751, G495259313, G4967125459, G4993796443, G5006930394, G5254703431, G6187336583, G6605821054, Health Outcomes from Raised Urban Settings, G678184136, G7396298195, G759215502, G7637048739, G7961497022, G7986115, G8107145774, G8113485484, G8134978103, Nuevas perspectivas en la relación mitofagia, autofagia y disfunción mitocondrial en la diabetes tipo 2: implicaciones clínicas y complicaciones cardiovasculares asociadas, G8343562339, G837542980, G8415626932, G8617628704, G8756105570, G8853716998
OpenAlex ID: W4409733260