Ultrasonication-Assisted Cell Lysis of Chicken Liver Cells for DNA Isolation and Adsorption-Elution Studies on CoFe2O4@ZnO
Year: 2024
Type: article
Abstract: Extracting nucleic acids for identification of suspects in the field of forensic science is still an arduous assignment and requires less intensive and labour free protocols. Liver tissue of mammals is known to contain high density of nuclei which leads to extraction of high amount of genetic material. Therefore, DNA extraction has been performed using newly developed lysis method with conventional method as well as the nanocomposites were brought in use to check the adsorption and elution capabilities. The developed magnetic nanocomposites CFO@ZnO were synthesized using solution combustion method and co-precipitation method and characterized using X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The newly developed CFO@ZnO nanocomposites show exceptional quality of DNA extraction by the revamped approach of lysing the cells as compared to the traditional method of cell lysis. This cutting-edge method is cost efficient as well as less time consuming with superior performance and thus proves to be an updated strategy of cell lysis.
Source: NFSU Journal of Forensic Science
DOI: 10.63633/2t55cf39
Authors: Aman Verma, Shreya Naik, Dr. Saroj Babar
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W4409737653