Year: 2025
Type: article
Abstract: Molecular genetic markers are regularly implemented in rabbit management for better breeding by selecting for specific genotypes. This study aimed to examine the diversity of the melanocortin-4 receptor gene in rabbits in Central Java, Indonesia. A total of 35 bucks were used in this study, comprising 10 New Zealand White, 11 Hyla and 14 Hycole. The DNA was extracted by Gene JET Whole Blood Genomic DNA extraction kit. Single Nucleotide Polymorphism (SNP) was detected by Polymerase Chain Reaction (PCR) and DNA sequencing. Hot carcass weight, cold carcass weight, reference weight and cut point 1-5 weight were among the parameters that were measured. This study showed that Melanocortin 4 Receptor (MC4R) genes were polymorphic, containing missense mutations and one SNP at 519G→A SNP 519GA had two alleles, A and G, with three genotypes (AA, GG, and AG). The rabbit population did not achieve HardyWeinberg Equilibrium (HWE) and showed a low level of genetic diversity as indicated by the heterozygosity results. The result of this study concluded that the frecuency AG as a genotype was higher than either GG or AA and A had a higher allele frequency than G. We recommended improving the validity of the data across various rabbit populations
Source: Buletin Peternakan
Authors: Firda Tasya Kamila, Mamat Hamidi Kamalludin, Sutopo Sutopo, Dela Ayu Lestari, Enny Tantini Setiatin, Ananda Dwi Agustine, Alfa Putri Latifa, Zata Nabilah, Syaddad Verahry Philco, Asep Setiaji
Institutions: Diponegoro University, Universiti Putra Malaysia
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $52
Funder: Universitas Diponegoro
Award: G485459999
OpenAlex ID: W4409752371