Work

Self-assembled nano-hybrid composite based on Cu/CuXO nanoflower decorated onto hBNNS for high-performance and ultra-sensitive electrochemical detection of CEA biomarker

Year: 2025

Type: article

Abstract: Synergistic combination of metal/metal oxide with semiconductor as nano-hybrid composites (NHC), exhibits unmatched potential for developing nano-biosensors with superior stability, sensitivity, and selectivity. In this study, we report the fabrication of hydrothermally synthesized 3D 2D NHC based on self-assembled Cu/Cu X O-hBNNS for label-free detection of Carcinoembryonic Antigen (CEA). A systematic investigation into the synthesis of Cu X O-NF, hBNNS , and Cu/Cu X O-hBNNS NHC was carried out using extensive spectroscopic and advanced nanoscale imaging techniques. Uniform deposition of Cu/Cu X O-hBNNS films onto pre-hydrolyzed ITO electrodes was achieved at a low DC potential of 15 V using electrophoretic deposition (EPD). Optimum immunoelectrode efficacy was analyzed by monitoring antibody incubation time , electrolyte pH, and control study through FTIR and electrochemical techniques . Electrode study revealed remarkably improved surface chemistry upon Cu/Cu X O integration with hBNNS , yielding ∼74 % and ∼ 31 % increase in CV and DPV response along with 3-fold increase in diffusion coefficient compared to bare hBNNS . The sensing response of the BSA/Anti-CEA/Cu/Cu X O-hBNNS/ITO nano-biosensor detected CEA concentrations from 0 to 50 ng/mL utilizing [Fe(CN 6 ) 3−/4- ] as a redox probe and demonstrated an exceptionally low limit of detection of 3.22 pg/mL (R 2 = 0.99998). Electrochemical clinical evaluation supported by ELISA test established that Cu/Cu X O-hBNNS-based nano-biosensor demonstrates exceptional shelf life, low cross-reactivity, and superior recovery rates in human serum, highlighting its effectiveness for precise and reliable detection.

Source: Bioelectrochemistry

DOI: 10.1016/j.bioelechem.2025.108993

Authors: Kanika Sharma, Nitin K. Puri, Bharti Singh

Institution: Delhi Technological University

Language: English

Topic: Electrochemical sensors and biosensors

Subfield: Electrical and Electronic Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,970